2.1.6.2.2 Elaboration Phase Iteration plan

Figure 1 Example of Iteration of the FlowDx Project.
Each iteration will look essentially the same but will have better understanding of the requirement and a more-complete set of results for all use cases and algorithms.

Goals for Elaboration Phase

2.1.5.2 Life Cycle Architecture Milestone (LCA) - Product's architecture is stabilized at the end of Elaboration Phase.

Use Cases

Collect the Use Case Data
Describe the Use Cases
Create SOPs for Gating
Have a set of Experts manually gate the data to create the expert consensus. (Gate-a-thon)

Algorithms

Test and validate the algorithms with a subset of the use case data

Metrics

Use Excel to calculate Match Ratio on Gate-a-thon results
Research the alternatives.
Identify other methods. (V-Measure [48], Mallows Distance [49], ROC [15], Misclassification rate [50], PCA)
Run first meta-analyses on all the different measures to find the principal components
Promote open descriptions of these methods to improve collaboration.

Phase Workproducts

Use Cases

Use Case Descriptions using MiFlowCyt
Gating Analysis SOPs
Initial Results

Algorithms

Initial Reports for each algorithm

Metrics

Initlal Results of March Ratio Calculations
Papers referenced.[48-53]
Fran's phython code to compare multiple metrics to each other.
Collaborators sign off

Elaboration Phase - Simplified Iteration Plan

Iteration #1
Use case data receipt, organization and posting to intranet, description, and gating instructions - Jill
Do Tree Star internal Gate-a-thon to get consensus gates at Tree Star April Meeting, TATSIS - Jill did prep, ApScis did gating
Elaboration of Match Ratio and presentation at FICCS meeting. - Jill
Manual Analysis of Match Ratio using spreadsheets. -Jill

Iteration #2
Explore Algorithms on some use case data. - Jill (Magnetic), Maciej (PBCA), John Quinn (ANN, SVM)
Run Summer Intern Program: FlowJo Newsletter - Jill with help from Susan, Tom, and Adam
Intern analysis collated by hand to get diagnostic decision/bioloigcally relevant results - Jill
Tree Star ApSci Summer Gate-a-thon analysis collated in the same way: SIV results. - Jill

Iteration #3
Build prototype repository and DB. Load all the FCS files. Done on Windows XP, Vista, and Mac. - Jay
Create reports of all the work.

Iteration #4
Build the repository and DB on Linux. Load all the FCS files and workspaces. - Ryan Minihan
Create the Comprehensive Project Plan- Adam, Jill, Tom, Eden
Submit the Phase II SBIR Grant Application to the NIH. -Tom, Jill, Adam, Eden
Iteration plan for first few iterations of Construction Phase 2.1.6.2 Iteration plan - Jill, Adam

As of Dec. 2009 everything has been completed except building the repository on Linux.

2.1.6.2.2 Elaboration Phase Iteration plan

Figure 1 Example of Iteration of the FlowDx Project. Each iteration will look essentially the same but will have better understanding of the requirement and a more-complete set of results for all use cases and algorithms.

Goals for Elaboration Phase

Algorithms

Metrics

Phase Workproducts

Algorithms

Metrics

Elaboration Phase - Simplified Iteration Plan

Figure 1 Example of Iteration of the FlowDx Project.
Each iteration will look essentially the same but will have better understanding of the requirement and a more-complete set of results for all use cases and algorithms.